Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 2. Recombinant VSIG4 activates the NRF2 signaling pathway to inhibit oxidative stress and inflammatory responses in

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: Recombinant

Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 5. The NRF2 inhibitor Brusatol inhibits the protective effect of VSIG4 in vitro. (A–C) Western blotting analysis of NRF2 and HO-1 in RAW264.7 cells exposed to Brusatol, n = 3. (D–F) Levels of GSH, SOD and MDA levels in Brusatol-exposed RAW264.7

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: In Vitro, Western Blot

Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 6. The NRF2 inhibitor Brusatol inhibits the protective effect of VSIG4 in vivo. (A) Neurological scores, n = 5. (B) Brain water content, n = 5. (C–G) Western blot analysis was conducted to measure the protein levels of NRF2, HO-1, Bcl-2, and Bax in brain tissues

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: In Vivo, Western Blot

Journal: Cell reports

Article Title: Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution

doi: 10.1016/j.celrep.2024.114911

Figure Lengend Snippet:

Article Snippet: anti-mouse NRF2 (D1Z9C) XP Rabbit mAb , Cell Signaling Technology , Cat#: 12721; RRID:AB_2715528.

Techniques: Purification, Plasmid Preparation, Produced, Recombinant, Concentration Assay, Saline, Labeling, Membrane, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Phospholipid Assay, BIA-KA, RNAscope, HD Assay, Polymer, Sequencing, Gene Expression, Software, Microscopy

Journal: Nutrients

Article Title: Ganoderma lucidum Polysaccharides Ameliorate Acetaminophen-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Apoptosis along the Nrf2 Pathway

doi: 10.3390/nu16121859

Figure Lengend Snippet: Antibody list.

Article Snippet: The antibodies including rabbit anti-mouse Nrf2, HO-1, GCLC, NQO1, and β-actin were purchased from ABclonal Technology Co., Ltd. (Wuhan, China), and goat anti-rabbit IgG HRP was purchased from Beyotime Biotech, Inc. (Shanghai, China).

Techniques:

Journal: Nutrients

Article Title: Ganoderma lucidum Polysaccharides Ameliorate Acetaminophen-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Apoptosis along the Nrf2 Pathway

doi: 10.3390/nu16121859

Figure Lengend Snippet: Primer sequence of qRT-PCR.

Article Snippet: The antibodies including rabbit anti-mouse Nrf2, HO-1, GCLC, NQO1, and β-actin were purchased from ABclonal Technology Co., Ltd. (Wuhan, China), and goat anti-rabbit IgG HRP was purchased from Beyotime Biotech, Inc. (Shanghai, China).

Techniques: Sequencing

Journal: Nutrients

Article Title: Ganoderma lucidum Polysaccharides Ameliorate Acetaminophen-Induced Acute Liver Injury by Inhibiting Oxidative Stress and Apoptosis along the Nrf2 Pathway

doi: 10.3390/nu16121859

Figure Lengend Snippet: Effect of GLP treatment on the expression of Nrf2 pathway-related genes and proteins: ( A ) Nrf2, HO-1, GCLC, and NQO1 mRNA expression levels in liver tissue; ( B ) quantitative analysis of protein expression ( n = 6); ( C ) protein imprinting analysis; compared with the normal group, ** p < 0.01; compared with the model, # p < 0.05, ## p < 0.01. Normal control: blank control group, model: model group, positive control: positive group, GLP-L: Ganoderma lucidum polysaccharide low-dose group, GLP-M: Ganoderma lucidum polysaccharide medium-dose group, GLP-H: Ganoderma lucidum polysaccharide high-dose group.

Article Snippet: The antibodies including rabbit anti-mouse Nrf2, HO-1, GCLC, NQO1, and β-actin were purchased from ABclonal Technology Co., Ltd. (Wuhan, China), and goat anti-rabbit IgG HRP was purchased from Beyotime Biotech, Inc. (Shanghai, China).

Techniques: Expressing, Control, Positive Control